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Hitachi L-8900 Service Manual
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Hitachi L-8900 Service Manual

High speed amino acid analyzer
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SERVICE MANUAL
FOR
L-8900 HIGH SPEED AMINO ACID ANALYZER
Hitachi High-Technologies Corporation
Copyright C Hitachi High-Technologies Corporation 2005.
All rights reserved. Printed in Japan.
Part No. 852-9051 TR-F (HT-HMS056003)

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  • Page 1 SERVICE MANUAL L-8900 HIGH SPEED AMINO ACID ANALYZER Hitachi High-Technologies Corporation Copyright C Hitachi High-Technologies Corporation 2005. All rights reserved. Printed in Japan. Part No. 852-9051 TR-F (HT-HMS056003)
  • Page 2 PREFACE This Service Manual is designed for Model L-8900 High Speed Amino Acid Analyzer, exclusively used for engineers approved by our company, to catch and diagnose trouble symptom and remedy the instrument as soon as possible. If trouble can be expected, remedy is easy, however, unexpected trouble may occur generally.
  • Page 3 IMPORTANT CE Conformity Marking This instrument, considering use in Europe, satisfies the following demands, has CE conformity marking. 1 EMC Demand This instrument conforms to EMC order 89/336/EEC, has CE conformity marking, is designed to safety EN61326 (1997) and its amendment 1 (1998), amendment 2 (2000).
  • Page 4 Precautions on Electromagnetic Disturbances Electromagnetic Interference by This Instrument to the Surroundings Operation of this instrument may cause electromagnetic interference on the electronic or electric equipment nearby such as a radio or TV set. Only those connection cables that are specified in the instruction manual are allowed to connect the instrument to configure your desired system.
  • Page 5 Electromagnetic Interference by the Surroundings to This Instrument Operation of this instrument in the vicinity of equipment that generates strong electric or magnetic fields may be responsible for a poor performance or malfunction of the instrument through noise induced by those strong fields. Only those connection cables that are specified in the instruction manual are allowed to connect the instrument to configure your desired system.

  • Page 6: Warranty On Product

    WARRANTY ON PRODUCT Model L-8900 Amino Acid Analyzer is guaranteed as follows, only when it is used following the contents of the instruction manual, based on the specifications described in the instruction manual. No guarantee is offered on express or implied quality, performance, merchandise value, or any fitness for special purpose to use.
  • Page 7 Failure due to use or use method not described in the instruction manual, due to repair not controlled by us. (g) Failure due to maintenance and repair performed by Maintenance Service company not specified by us. (h) Failure due to relocation or transport not approved by the manufacturer after initial installation.

  • Page 8: Installation/Relocation

    INSTALLATION/RELOCATION Customers should not performed installation at the delivered. Engineers who have been trained by our service department or our company, and approved by our company perform installation so that the instrument will be used safety and precisely. DISPOSAL Matters directly affect environment are not used. However, laws or regulations may be changed in the future.

  • Page 9: Safety Summary

    SAFETY SUMMARY Before using the Model L-8900 High Speed Amino Acid Analyzer, be sure to read the following safety instruction carefully, and understand the contents completely. General Safety Guidelines • When operating, follow instructions in the instruction manual for each instrument.
  • Page 10 SAFETY SUMMARY General Safety Guidelines (Continued) In addition, the following signal word is used for ensuring proper use of the product. NOTE : Used to indicates in instruction for ensuring correct trouble-free use of the instrument and accurate measurement therewith. SAFETY - 2...
  • Page 11 SAFETY SUMMARY General Safety Guidelines (Continued) Precautions on Use Before using this instrument, be sure to read the instruction • manual carefully to attain a solid understanding of its functions and operations. Keep the instruction manual handy so that it can be referred •...
  • Page 12 SAFETY SUMMARY General Safety Guideline (Continued) Precaution during Use If an abnormal condition such as unusual noise, odor, fuming or gas leakage occurs during operation of the instrument, disconnect the power cable immediately. Provide proper safety measures as required, contact our Service office. Precautions on Installation, Maintenance and Relocation •...

  • Page 13: Danger Indications

    SAFETY SUMMARY Safety Instructions in This Manual Shown below are the safety instructions contained in this manual and their relevant sections in it. DANGER Indications DANGER The indication " " does not apply to this instrument. WARNING Indications Warning: Burst Handling Reagent Containers •...
  • Page 14 SAFETY SUMMARY WARNING Indications (Continued) Warning: Infection Protective tools described below are Plastic gloves for preventing infection, safety goggles, Protective gown, and Protective mask. Handling Bio-Samples If touching samples, you may be infected with infective matters in samples. Liquid leaked from the flow path may cause infection. Wear Protective tools when handling samples.
  • Page 15 SAFETY SUMMARY WARNING Indications (Continued) Warning: Ignition of Flammable ChemicalsWarning: Infection Handling Flammable Chemicals When using flammable chemicals such as organic solvents, ignition may occur. Always check the following. If abnormal, stop using at once. • Solvents, Waste solutions should not leak. •...
  • Page 16 SAFETY SUMMARY WARNING Indications (Continued) Warning: Ignition of Flammable Chemicals by Static Electricity Handling Flammable Chemicals When using Flammable Chemicals such as organic solvents, Ignition can occur. When using Flammable Chemicals, be careful about Ignition by Static Electricity. To prevent ignition by Static electricity, pay attention to the following.
  • Page 17 SAFETY SUMMARY WARNING Indications (Continued) Warning: Electric Shock due to Improper Grounding Improper Grounding may cause Electric Shock Hazard Be sure to use Power Cable furnished with the instrument. Power Cable other than the specified may cause electric shock. This instrument is 'Plug connection-type equipment' (European standard EN61010-1).

  • Page 18: Caution Indications

    SAFETY SUMMARY CAUTION Indications Caution: Burns Column Oven Touching Column Oven may cause burns of high temperature (85 °C). When replacing Column, temperature display should be 55 °C or lower. Light Source Lamp Touching Lamp or Lamp Cover may cause burns of high temperature (70 °C).

  • Page 19: Caution

    SAFETY SUMMARY CAUTION Restriction of Reagent • For the flow path of eluant of this instrument, Fluorocarbon polymers, stainless steel (SUS316), ceramics, ruby, sapphire, quartz are used. Do not use solvents that corrode these materials. Use only solvents specified in the instruction manual.
  • Page 20 SAFETY SUMMARY When Stopping Instrument Manually • When stopping the instrument manually, choose one of the following. (a) Remove Sample vials from Autosampler, Start operation with Analysis program in use. The instrument performs analysis finish action at once. (After cleaning the flow paths, automatically stops in 1 hour.) (b) Stop ninhydrine pump (Pump 2), set temperature of Reaction device at OFF, run Buffer solution pump (Pump...
  • Page 21 When turning off the power of the personal computer, first finish EZChrom Elite for Hitachi AAA, Environment setting software, then, shut down the basic software.
  • Page 22 SAFETY SUMMARY About Computer Virus • If the program or data is destroyed suddenly, unexpected functions are displayed on the screen, the personal computer may be infected with computer virus. Computer virus is a bad program that invades a personal computer secretly, makes the personal computer function selfishly, destroys data freely.

  • Page 23: Warning Labels

    SAFETY SUMMARY Warning Labels The following Warning Labels are attached to Model L-8900 High Speed Amino Acid Analyzer. Read the Labels carefully, check the contents on the actual Labels. Check these Labels sometimes, clean and maintenance them so that they can be read well from a safe distance.

  • Page 24: Table Of Contents

    CONTENTS PREFACE ABOUT THIS MANUAL IMPORTANT ............ IMPORTANT - 1 CE Conformity Marking ........IMPORTANT - 1 Precautions on Electromagnetic Disturbances ......IMPORTANT - 2 Warranty on Product ........IMPORTANT - 4 Installation/Relocation ........IMPORTANT - 6 Disposal ............IMPORTANT - 6 Other Cautions ..........
  • Page 25 4.2 MAINTENANCE SPARE PARTS ........ 4 - 2 4.3 COLUMN REPLACEMENT ......... 4 - 3 4.4 L-8900 PART, PART NUMBER LIST ......4 - 4 4.5 UNIT PARTS REPLACING PROCEDURE ....4 - 5 4.5.1 Column Oven ............4 - 5 4.5.2 Reaction Bath ............

  • Page 26: Outline

    (d) Detection sensitivity: 3 pmol (S/N = 2, Asp) Analysis unit (a) Column: Size: 4.6 mm × ID 60 mm Note 1) Resin: Hitachi custom ion exchange resin Note: 1: Inch-screws (Unify) are used. (b) Pump: Discharge pressure: 0 - 30 MPa (0 - 306 kgf/cm Flow rate: 0.000 - 0.999 mL/min...

  • Page 27: Comparison With Model L-8800A

    1.2 COMPARISON WITH MODEL L-8800A 1.2.1 Comparison of Specifications Comparison of specifications and performances between Model L- 8900 and Model L-8800A is shown in the table below. Item Model L-8900 Model L-8800A Personal computer Windows XP Windows 2000 Professional Professional...
  • Page 28 Item Model L-8900 Model L-8800A Sampler cleaning liquid (C1) Degasser Degassing pipe degassing Leak sensor Yes, used. No, not used. Column Oven Temperature control method Electron thermal control Electron thermal control 20 - 85 °C (1 °C step) 20 - 70 °C (1 °C step)
  • Page 29 Item Model L-8900 Model L-8800A RG process Yes. (Edition possible) Yes. (fixed) Programming of RG process can be edited freely. Cleaning process Yes. Yes. Module operation Manual operation Module operation Data process Peak ratio diagnosis function No, not provided. Yes, provided.
  • Page 30 Item Model L-8900 Model L-8800A System conformity test calculation method Area/Height method Theoretical plate Theoretical plate number number Choice factor Choice factor Capacity factor Capacity factor Symmetry factor Symmetry factor Separation degree Separation degree Theoretical plate height Data diagnosis Diagnosis peak (Area Diagnosis peak (Area value, SST, etc.)

  • Page 31: Configuration Of Instrument

    1.3 CONFIGURATION OF INSTRUMENT 1.3.1 Inside Configuration Degasser for C1 gas unit Column oven Autosampler cleaning Photometer Line filter liquid (C1) Autosampler Sampler cooling device Mixer 1 (option) Pump unit 1 (for buffer solution) Reaction device Automatic purge value (Full specification version) Container for buffer solution, reaction solution...

  • Page 32: Pump Unit

    1.3.2 Pump Unit Ammonia filter column Pump head 1 Pump head 2 Discharge valve Discharge valve Drain valve Pump head 1 Pump head 2 Drain valve Automatic purge valve Flow path filter Suction valve Suction valve (Full specification version) Automatic purge valve (Full specification version) Pump 1 Pump 2...

  • Page 33: Autosampler

    1.3.4 Autosampler Cleaning port Cleaning liquid (C1) Rack Syringe valve Drain Syringe 1 - 8...

  • Page 34: Flow Path System

    1.4 FLOW PATH SYSTEM The flow path system of Autosampler is shown in Figure 1-2. Sampler vials are set on the sample rack. The needle can move to the sample vial, cleaning port and injection port by moving the sample rack in the right-left direction (X-direction), and the needle arm in the front-back direction (Y- direction) and in the vertical direction (Z-direction).

  • Page 35: Analysis Method (For Details For The Analysis Method, See Chapter 4 Of The Instruction Manual.)

    Net: 60 min ± 3 min Analysis time Cycle: 75 min ± 3 min 2. Specifications for reagent No. Item Contents Part number 855-4340 L-8900 PFS kit Contents PFS-1: 2 pieces PFS-2: 2 pieces PFS-3: 1 piece PFS-4: 1 piece PFS-RG: 1 piece...
  • Page 36 (Amino acid mixture standard solution by Wako Reagent Industry Co.) Buffer solution See 2. Specifications for reagent. Analysis file (method) See separate data. 4. Products used Model L-8800A, Model L-8900 High-speed physiological fluid analysis Retention time (min) 1 - 11...

  • Page 37: High Separation Physiological Fluid Analysis Method

    1.5.2 High Separation Physiological Fluid Analysis Method 1. Specifications for analysis column and analysis method No. Item Contents Part number 855-4350 6.0 mm ID × (25 × 25) mm Column size Packing material Cation exchange resin #2622 (Li type) Separation degree JP Thr/Ser: 1.2 or more Gly/Ala: 1.2 or more Ile/Leu: 1.0 more...
  • Page 38 2 nmol/20 µL (Amino acid mixture standard Water solution sample solution by Wako Reagent Industry Co.) Buffer solution See 2. Specifications for reagent. Analysis file (Method) See separate data. 4. Products used Model L-8800A, L-8900 High separation physiological fluid analysis Retention time (min) 1 - 13...

  • Page 39: Unpacking, Installation

    2. UNPACKING, INSTALLATION 2.1 UNPACKING Unpack the shipping box being careful not to give shocks to the instrument, take out gently the instrument, and put it on a solid and flat place. Set down the casters on the front of the instrument so that the instrument will not move.

  • Page 40: Environment Of Installation Place

    900 mm 700 mm Power supply Ground 2.5 m PC set table or Model L-8900 PC rack main unit When PC is set on the main unit, this space is not required. Figure 1-2 Installation Space 2.2.2 Environment of Installation Place...

  • Page 41: Other General Cautions

    (4) Others (a) No direct sunlight (may affect the performance, color of the instrument). Avoid placing near the window. (b) No strong vibrations or shocks that can be felt by you. (Fine adjustment mechanism is affected.) (c) No heat generators such as gas burner, heater, or oven nearby.
  • Page 42 Table 1-1 Necessary Items Item Specification Quantity Remarks (Use) gas cylinder Purity: 99.99 % or higher gas regulator Secondary regulating valve full scale: 6 kgf/cm (0.6 MPa) or less. Beaker 50 mL 500 mL Measuring 100 mL cylinder Haul pipette 0.5 mL 1 mL 2 mL...

  • Page 43: Installation Procedure

    2.3 Installation Procedure Reagent Setting 1a. Turn on the power, and make sure initializing function of each Module is completed. 1b. Start EZChrom to display Instrument Status screen. Click L-8900 tab. Click Connection Execution button of the system to initialize. １ ２...
  • Page 44 1c. Make sure that the regulator of the main unit is OPEN, and the pressure display is 0 kPa. 1d. Make sure the fixings of the Sampler are removed. 1e. Rinse the solvent filter for each reagent with distilled water, make sure no dust stuck to it, wipe it with a cleaning paper to remove water, put it in the reagent bottle, and set the reagent.
  • Page 45 ３ ２ ４ Pump Purge Display the Instrument Status screen, click L-8900 tab. 3a. Pump purge 3a1. Click Pump 1 Setting button of Manual operation, set the flow rate at 0.999 mL. 3a2. Input 25 % each for the ratio of B1, B2, B3, B4, click Execution button.
  • Page 46 OPEN 3a3. Open Drain valve. 3a4. Turn on the Pump 1 switch. Click Pump 1 switch of Manual On/Off, Click ６ OK on the Pump 1 Windows screen. 3a5. Perform purge for about 30 minutes. 3a6. Turn off the Pump 1 switch. CLOSE Click Pump 1 switch of Manual On/Off, click...
  • Page 47 OPEN 3b3. Open Drain valve. 3b4. Turn on the Pump 2 switch. Click Pump 2 switch of Manual On/Off, ５ click OK on the Pump 2 Window screen. 3b5. Perform purge for about 20 minutes. 3b6. Turn off the Pump 2 switch. Click Pump 2 switch of Manual On/Off, CLOSE...
  • Page 48 4g. Connect a piping to the inlet side of Ammonia filter column. 4h. Turn on the Pump 1 again. 4i. Pump liquid until bubbles do not come out from the outlet side of Ammonia filter column. 4j. When no bubbles come out, stop the Pump 1. 4k.
  • Page 49 Connecting Separation Column * The Separation column is removed before shipment like the Ammonia filter column. 5a. Set the flow rate of Pump 1 at 0.3 mL. Set the ratio of B1 at 100 %. Turn on the Pump 1. At this time, the Separation column is not connected yet.
  • Page 50 5i. After connection, clean the part where reagent has been stuck. Removing Bubbles form the Syringe Remove bubbles from the syringe as follows. 6a. Remove the sealing screw of Port 5 of Syringe valve. Syringe valve Sealing screw Syringe 6b. Connect the furnished plastic injector to Port 5. Or connect a tube of about 0.25 mm ID ×...
  • Page 51 6c. Display the Instrument Status window, and click Autosampler of Manual operation. Choose Pump cleaning, and click Execution button. 500 L of cleaning solution is sucked and discharged to Port 5. ２ １ ３ 2 - 13...
  • Page 52 When the plastic injector is used, at timing of discharging the cleaning solution to Port 5, press the Syringe with your fingers to give proper pressure (several times) to remove bubbles easier. Notice： If bubbles in the Syringe are not removed easily, remove the Syringe (see 6.3.8), clean the inside of Syringe with isopropanol, then try removing bubbles from the Syringe.
  • Page 53 6e. Choose Home, and click the Execution button. 6f. Choose Sampler Cleaning, and click the Execution button. Replace the cleaning solution in the Cleaning port. Setting Temperature of Oven and Turning Lamp on On the Instrument Status screen, set temperature of Column Oven at 50 °C, and temperature of Reaction bath at 135 °C to execute.
  • Page 54 Editing Method 8a. Create Method to be used for installation through 'File' → 'Method' → 'Open'. Choose 'Standard analysis_met' from the Method file to open. 2 - 16...
  • Page 55 8b. Open 'Method' → 'Instrument conditions'. 8c. Click L-8900 tab, select name of column from Pull-down Menu. Change of reagent, Change of Gradient Program are necessary, perform edition on this screen. * When changing name of column, use the Maintenance software.
  • Page 56 8d. Click the Auxiliary trace tab, put a Check mark to Pump pressure, temperature. * By using the Monitor display, pressure variation of Pump, and temperature precision can be checked. １ 8e. Click the Baseline Check tab, and set the Baseline check time at 1 minute. Set the Baseline check time finishing time at 1 minute.
  • Page 57 8f. Click the Trigger tab, and make sure the type is 'External'. 8g. Save with a different name through 'File' → 'Method' → 'Name and save'. 2 - 19...
  • Page 58 Creating Sequence File 9a. Open 'File' → 'Sequence' → 'Sequence wizard'. 9b. Click File development button. １ 2 - 20...
  • Page 59 9c. Choose Method to be used, click Open button. １ 9d. Check that the Method has been changed to the chosen Method, and click Next button. １ 2 - 21...
  • Page 60 9e. Input Installation <D> for Sample ID. Input Installation <D> for Data file. Input 8 for number of unknown samples, and click Next button. １ ２ ３ ４ 9f. Input 20 for Autosampler injection volume, and click End button. １ ２...
  • Page 61 9g. Input The same number for all the vials. Input 0 for the injection volume of the first line and the second line. Change the Method of the first line to RG Method. ２ ３ ４ １ Click Analysis type pull-down menu of the third line, change the Analysis type to Baseline Check, and click OK button.
  • Page 62 Move the cursor to the right edge of the sequence, and click Action pull-down menu of the third line. ７ Choose Baseline check from the Test column, Fail from the Result column, Continue from the Action column, and click OK button. ９...
  • Page 63 BLC and save the sequence. 'File' → Make sure Action has been changed to 'Sequence' → 'Name and save'. １２ 10. Analysis Start Pump 1 and Pump 2 before starting analysis. Make sure temperature of the Reaction bath is at 135 °C, then start measurement. On the Instrument Status screen, •...
  • Page 64 10a. Preparation for analysis Start the instrument with 'Single run'. While the instrument is in idling, click Single run icon. １ Input Installation preparation <D> for Sample ID. Input Installation preparation <D> for Data file. Input 1 for number of repetition, input 0 for injection volume.
  • Page 65 10b. Addition of run During preparation for analysis by Single run, add Sequence run. During Single run measurement, click Sequence run icon １ Click File Development button, and choose Sequence for installation. After choosing Sequence, click Addition button of run. ３...
  • Page 66 After adding Sequence, click Run queue icon , and check that it has been added to Run queue. ４ ５ 2 - 28...
  • Page 67 11. Creating Report Template 11a. Start Template editor through 'File' → 'Detailed report' → 'New creation'. Input Report name into the cell of the first line or the second line. Right-click the cell of the third line onward, choose Table wizard. １...
  • Page 68 11c. Set parameters of Table wizard. Move the parameters of Retention time, Area, Resolution from the left-hand box to the right-hand box on the screen, click Next button. * Set 0 for the decimal point fingers of only Area. １ 11d.
  • Page 69 11e. Set Execution parameters of Table wizard. Move the parameter of Sample ID from the left-hand box to the right-hand box on the screen, click Next button. １ 11f. Set Execution direction of Table wizard. Put a Check mark for horizontal direction, and click End button.
  • Page 70 11g. Save the created Report template. Name and save through 'File' → 'Detailed report' → 'Save'. 12. Data Process 12a. Display the chromatograph of measured data through 'File' → 'Data' → 'Open'. Display the data. If retention time of each ingredient is shifted, or the waveform process is not appropriate, perform edition of Peak/Group table/Integration table.
  • Page 71 12b. Click 'Method' → 'Integration table'. If the waveform process is not appropriate, change Event, change Time or Value. １ ２ ３ 12c. Click 'Method' → 'Peak/Group'. If the retention time is shifted, see the data, and correct Retention time of each ingredient.
  • Page 72 13. Re-analysis 13a. Start EZChrom by the desktop icon. Right-click on the instrument icon and open 'Exclusive analysis'. 13b. Creating Sequence file for re-analysis. Click 'File' → 'Sequence' → 'Sequence wizard'. 2 - 34...
  • Page 73 13c. Click File development button, and choose 'Method' used for measurement. Already existing (for analysis) for the kind of Data file, and click Next button. １ ２ ３ 13d. Click File development button to choose Data file. １ 2 - 35...
  • Page 74 Choose 6-samples of measured data file, and click Open button. ３ ２ Make sure the measured data have been chosen in the Data file box, and click End button. ４ 2 - 36...
  • Page 75 13e. Click Analysis type pull-down menu of the first line, change Analysis type to Summary start (SMB), change Report template to Reproducibility at installation, and click OK button. １ Change the second line through the fifth line to Summary (SMR). Change the sixth line to Summary end (SME).
  • Page 76 13f. When Re-analysis sequence has been created, name and save it. 13g. Click 'Sequence' → 'Analysis'. 2 - 38...
  • Page 77 13h. Click the folder to select Re-analysis sequence, and click Start button. 13i. When Analysis is completed, click 'Report' → 'Display' → 'Sequence report'. 2 - 39...
  • Page 78 13j. When Summary report is created for Sequence report, choose it, and click ① View button. １ 13k. Check the result. When installation performances are satisfactory, click Print button. 2 - 40...

  • Page 79: Troubleshooting

    3. TROUBLESHOOTING 3.1 TROUBLE CAUSE ANALYSIS 3.1.1 Pump Pressure Abnormal Result Trouble Check Point Cause Check Remedy Remarks Solenoid valve (R1 1. Reaction column should be replaced. Replace Reaction side) for ninhydrine Remove Column outlet Teflon Contact our Service office. column.

  • Page 80: Baseline Noise

    Result Trouble Check Point Cause Check Remedy Remarks Bubbles generated Open Drain valve and purge. in Pump valve. Dirty Pump valve Disassemble and clean Suction valve, Pump unit (common) (Seal dust). Discharge valve, and pump head. See 6.3.1 or 6.3.5 for cleaning way. Replace Pump seal (life is about 1 month).

  • Page 81: Baseline Drift

    3.1.3 Baseline Drift Result Trouble Check Point Cause Check Remedy Remarks Leakage Use a wrench for tightening joints to which wrench available pushing screws are used. However, Drift (including wave) Flow path joint Check visually, tighten leaking joints. for other joints, especially Teflon tube joints, use only your hands. (Do not use tools.) (Or, Teflon tubes are crushed to cause clogging.) Run Pump 1 and Pump 2 in this order, wait for 30 Warning up insufficient...
  • Page 82 3.2 TROUBLESHOOTING 3.2.1 Troubleshooting of Units (common) 1. Error messages No. Symptom Contents Remedy ROM error occurred in PC board malfunctions. Turn off and on the power. ***. Turn off the power of Instrument and start again. RAM error occurred in PC board malfunctions.
  • Page 83 3.2.2 Troubleshooting of Autosampler 1. Troubleshooting Table No. Symptom Cause Remedy Poor 1. Air exists in the flow path 1. Perform Wash operation reproducibility system. (Syringe, tubing several times to purge air. etc.) Clean the flow path system. 2. Leakage from Injection port. 2.
  • Page 84 No. Symptom Cause Remedy There is carry- 1. Dirty flow path system. 1. Clean the flow path system. over. 2. Leakage from Injection port 2. Check and tighten the Lock seal. nuts. Replace the Seal. 3. Dirty container of Cleaning 3.
  • Page 85 2. Error messages (Autosampler) No. Symptom Contents Remedy Vial detection error Vial detection was done Set sample vials. occurred in incorrectly. Autosampler. 'Rack loader motion: X 1. Sampler rack or Sample vial 1. Set Sample rack and motor' error occurred in is caught.
  • Page 86 3.2.3 Troubleshooting of Pump 1. Error messages No. Symptom Contents Remedy 'Pressure upper limit' Pressure became higher than Check leakage and error occurred in ***. the upper limit. clogging. 'Pressure lower limit' Pressure became lower than the Check leakage and error occurred in ***.
  • Page 87 3.2.4 Troubleshooting of Column Oven/Reaction Bath 1. Error messages No. Symptom Contents Remedy 'Temperature lower Temperature sensor abnormal. Turn off and on the power. limit value abnormal If error occurs again, (Oven side)' error contact our Service office. occurred in Column oven.
  • Page 88 3.2.5 Troubleshooting of N Gas Unit 1. Error messages No. Symptom Contents Remedy gas PC board PC board, wiring abnormal. Turn off and on the power. connection' error If error occurs again, occurred in N2 gas contact our Service office. unit.

  • Page 89: Pump

    4. TECHNICAL INFORMATION 4.1 MAINTENANCE PARTS LIST Always prepare the following regular replacing parts as many as necessary for the operation. Expecting life and necessary quantity for 1 year are based on standard analysis and full operation of 300 days. These values depend on operating conditions greatly. Prepare necessary quantity considering actual operating conditions.

  • Page 90: Maintenance Spare Parts

    4.2 MAINTENANCE SPARE PARTS Prepare the following parts when operating the Instrument for a long time. Part number Part name Used for Quantity to Remarks keep 855-5028 Plunger Pump 1, 2 885-2920 Drain valve seal Drain valve 810-1128 Drain valve packing Drain valve 855-5084 Check valve unit...

  • Page 91: Column Replacement

    4.3 COLUMN REPLACEMENT Column are articles of consumption. Replacing timing is roughly as follows. These values greatly depend on pre-treatment status of sample, interfering matters contained is sample (solid matters, oils and fats, protein, etc.). Repacking is possible as well as replacing. These values described here are not guaranteed values.

  • Page 92: L-8900 Part, Part Number List

    4.4 L-8900 PART, PART NUMBER LIST Unit Part name L-8900 L-8800A L-2000 Series Pump Pump seal 855-5030 855-5030 890-1130 (L-2100) 655-1080 (L-2130) Cleaning seal 855-5034 855-5034 855-5034 (L-2100) 885-1884 (L-2130) Plunger 855-5028 855-5028 890-1128 (L-2100) 890-1125 (L-2130) Pump head 1...

  • Page 93: Unit Parts Replacing Procedure

    4.5 UNIT PARTS REPLACING PROCEDURE 4.5.1 Column Oven Peltier replacing procedure 1a. Turn off the power of Model L-8900 main unit, disconnect the power cord. 1b. Remove the right side cover of the main unit. 1c. Remove the wiring connector from the PC board, remove it from the fixing clamp.
  • Page 94 Note： When removing Column oven unit, pull the harness to the neighborhood of Column oven unit beforehand, that makes pulling work easier. 1f. Remove Fan Base plate (P/N 852-3006) from Column oven unit (4 Fixing screws). Fixing screws (Upper and lower two positions) Fan base plate 1g.
  • Page 95 1h. Remove Teflon packing. (4 Fixing screws) Teflon packing Fixing screw (×4) 1i. Remove Column Holder assembly (P/N 852-3020) from the right/left Heat Sink (P/N 852-3014). (When removing Column Holder assembly, loosen the 8 Fixing screws one by one, little by little.) Remove Fixing screw.
  • Page 96 1j. Place the removed Column Holder assembly (P/N 852- 3020) facedown, remove the fixing plate for Thermal Protector, and remove Peltier assembly (P/N 852-3009). Peltier assembly Groove Thermal protector fixing plate Column Holder back surface 1k. Attach a new Peltier assembly. Apply Thermal conductive grease (P/N J379090) thin and evenly to the right surface and the reverse surface of Peltier.
  • Page 97 2. Temperature sensor for Oven replacing procedure 2a. Perform 1a - 1j of Peltier replacing procedure. 2b. Pull out the Temperature sensor for Oven, and attach a new Temperature sensor (P/n 852-3019). Temperature sensor for Oven Expanded 2c. Remove the remaining Thermal conductive grease after pulling out Temperature sensor for Oven, apply Thermal conductive grease (P/N J379090) enough, seal the outside with Silicone compound (P/N F375003), then, dry...

  • Page 98: Reaction Bath

    3e. Assemble in the reverse order of disassembly. 4.5.2 Reaction Bath Heater replacing procedure 1a. Turn off the power of Model L-8900. Disconnect the power cord. 1b. Remove the right side cover of the main unit. 1c. Remove the wiring connector from the PC board, and remove it from the fixing clamp.
  • Page 99 Remove Fixing screw. (×4) 1e. Remove Fan Base plate (P/N 852-3504). (4 Fixing screws) Remove Fixing screw. (×4) 1f. Pull Heater apart from Reaction bath. Heater assembly 4 - 11...
  • Page 100 1g. Apply Silicone compound (P/N 852-3526) to the surface of a new Heater assembly (P/N 852-3519) thin and evenly, and attach it to Heat Block (P/N 852-3514). Then, dry it for 3 - 4 hours. Apply Silicone compound thin and evenly to the surface of Heater assembly.
  • Page 101 2c. Remove 4 Fixing screws of Heat Block 1 (P/N 852-3514), remove it together with heat insulation material from Reaction bath cover. Remove 2 screws of the opposite side, total 4 screws. 2d. Remove the Heat insulation material, pull out Oven temperature sensor from Heat Block 1 (P/N 852-3514), attach a new Temperature sensor (P/N 852-3518).
  • Page 102 Heater temperature sensor replacing procedure 3a. Perform 2a - 2c of Oven temperature sensor replacing procedure. 3b. Pull out Heater temperature sensor, attach a new Temperature sensor (P/N 852-3518). Heater temperature sensor attaching position 3c. Apply enough thermal conductive grease (P/n J379090) to the circumference, seal the outside with Silicone compound (P/N 852-3526), then , dry it for 3 - 4 hours.

  • Page 103: Degasser

    4.5.3 Degasser Degasser electric system unit replacing procedure 1a. Remove 3 Fixing screws of N gas unit, pull the unit toward you. Note： Remove C-1 piping and Piping from Syringe valve 1. Remove Fixing screw. (×3) 1b. Remove Power cable of N gas unit from the PC board.
  • Page 104 1c. Remove 3 Fixing screws of Degasser electric system unit (P/N 852-2901). Note： When removing Electric system unit, remove N piping (blue) too. Remove Fixing screw. (×3) Degasser electric system unit 1d. Remove Piping of Degasser chamber for C1, remove Fixing screw (one piece) to remove Degasser chamber.
  • Page 105 1e. Remove Fixing screws (2 pieces + 1 Thumbscrew) of Vacuum chamber unit (P/N 852-2902), shift the Vacuum chamber unit toward you, fix only the left side by using the removed Thumbscrew. Remove Fixing screw. (×2) Vacuum chamber unit Fix with Thumbscrew. 1f.
  • Page 106 Vacuum chamber unit replacing procedure 2a. Remove Fixing screws (2 pieces + 1 Thumbscrew) of Vacuum chamber unit (P/N 852-2902), shift the Vacuum chamber unit toward you, fix only the left side by using the removed Thumbscrew. Remove Fixing screw. (×2) Vacuum chamber unit Fix with Thumbscrew.
  • Page 107 2c. Remove Pipings (IN/OUT) connected to each Solenoid valve. (2 Fixing screws each) Remove Fixing screw. (×2) 2d. Remove Manifold (P/N 852-4320). (2 Fixing screws) Remove Fixing screw. (×2) 2e. Replace Vacuum chamber unit with a new one. Assemble in the reverse order of disassembly. 4 - 19...

  • Page 108: Pump Purge Valve

    4.5.4 Pump Purge Valve Turn off the power of Model L-8900. Disconnect the Power cord. Pull out Pump unit. Remove Purge valve unit. (3 Fixing screws) Remove Fixing screw. (×3) Remove 3-way valve (P/N 852-8110) (4 Fixing screws). Replace the 3-way valve with a new one.

  • Page 109: Weight Sensor

    4.5.5 Weight Sensor Weight sensor for B1 - B6, R1 - R3. 1a. Turn off the power of Model L-8900. Disconnect the Power cord. 1b. Remove the right side cover of the main unit. 1c. Remove Fixing screws (2 pieces) of Receiver.
  • Page 110 1e. Remove 4 Fixing screws of Load Cell (30) assembly (P/N 852-8000), pull the Load Cell (30) assembly backward to remove. Remove 2 screws near you. Remove 2 screws at the opposite side, total 4 pieces. 1f. Attach a new Load Cell (30) assembly in the reverse order of removing.
  • Page 111 Weight sensor for C1 2a. Remove 2 Fixing screws of Cleaning Base (P/N 852- 1063), pull Cleaning Base together with Weight sensor for C1 toward you. Remove Fixing screw. (×2) 2b. Remove 2 Fixing screws of Receiver. Remove Fixing screw. (×2) 4 - 23...
  • Page 112 2c. Remove the wiring connector (J2) from AMP PC board (P/N 852-6042). Remove Connector (J2). 2d. Remove Load Cell (30) assembly (P/N 852-8000). (4 Fixing screws) Remove Fixing screw. (×4) 2e. Attach a new Load Cell (30) assembly in the reverse order of removing.
  • Page 113 Adjustment specifications for 30N type Load Cell. 3a. Switching PC board switches 3a1. Set SW1 as follows. R1, R2 B1 - B6 Cleaning solution * Connector of AMP PC board for R1 and R2 has one red circle as a mark. 3b.

  • Page 114: Weight Sensor For Waste Solution

    4.5.6 Weight Sensor for Waste Solution Remove 4 Fixing screws of Receiver. Remove Fixing screw. (×4) Remove 4 Fixing screws of Load Cell (100) assembly (P/N 852-8001). Remove Fixing screw. (×4) Remove the wiring connector (J2) from AMP PC board (P/N 852-6042).

  • Page 115: Adjustment Specifications For 100N Type Load Cell

    4.2.7 Adjustment Specifications for 100N type Load Cell Switching PC board switches 1a. Set SW1 as follows. Waste solution * Connector of AMP PC board for waste solution has two red circles as marks. Adjusting Load Cell lower limit stopper 2a.

  • Page 116: Adjusting Pc Board

    4.6 ADJUSTING PC BOARD 4.6.1 Adjusting ZERO/SPAN of PUMP PC Board (P/N 852-6010) Items to be prepared; UI pad for adjustment (P/N:852-8501) Pressure gauge (for reference) Connect UI pad to PUMP PC board. Connect Pressure gauge (for reference) to the outlet of Drain valve assembly.
  • Page 117 SPAN adjustment Pump liquid so that pressure monitor value of Instrument status in EZChrom will be 18.6 - 19.6 MPa (190 - 200 kgf/cm ), keep the conditions, adjust VR2 so that pressure display will be within (Actually measured pressure value) MPa.

  • Page 118: Adjusting Reaction Bath Device Pc Board (P/N 852-6033)

    4.6.2 Adjusting Reaction Bath Device PC Board (P/N 852-6033) Items to be prepared; UI pad for adjustment (P/N: 852-8501) Pseudo temperature load resistor: 123.24 (corresponding to 60.0 C) Pseudo temperature load resistor: 149.94 (corresponding to 130.0 C) Adjusting temperature for Oven Connect UI pad to ZJ1 of OVEN FP PC board (P/N 852-6033).

  • Page 119: Adjusting Column Oven Pc Board (P/N 852-6030)

    Connect the resistor of 149.94 (corresponding to 130.0 C) to ZJ6. Turn on the power of the main unit, make sure the following Initial screen is displayed on the UI pad. TEMP: MONITOR READY: RANGE LRAMP [ - ] 25.1 39.0 –...
  • Page 120 TEMP: MONITOR READY: RANGE LRAMP [ - ] 25.1 39.0 – 41.0 Adjust VR1 (OFFSET) of Reaction device PC board so that display value of Monitor will be within 20.0 0.2. Turn off the power of the main unit, connect the resistor of 123.24 (corresponding to 60.0 C) to ZJ4.
  • Page 121 Turn off and on the power of the main unit, display the above screen by 5 procedure, make sure display value of Peltier is within 60.0 0.2. Turn off the power of the main unit. Connect Temperature sensor for Peltier to ZJ6. Adjusting temperature for room temperature Remove Short Pin connected to ZJ5 connector of Column oven PC board.

  • Page 122: Handling Ui Pad, Button Function Correspondence

    4.7 HANDLING UI PAD, BUTTON FUNCTION CORRESPONDENCE Service function Pump Oven Reaction bath Annex Board Parameter initialization Press UTLTY key, 80 ENT Press UTLTY key, 80 ENT Press UTLTY key, 80 ENT Press UTLTY key, 80 ENT Press UTLTY key, 80 ENT LED test mode Press UTLTY key, 81 ENT Press UTLTY key, 81 ENT...

  • Page 123: Annex Board Ui Pad Operation

    4.7.1 Annex Board UI Pad Operation 1. Key arrangement VALVE1 VALVE2 ZERO PURGE SET UP ▲ ▼ UTILTY 2. LED Green: Power On Orange: Error, Light Off: Power Off 4 - 35...
  • Page 124 3. Monitor screen First Monitor screen (Lamp/gas monitor display) LAMP ENERGY GAS MONITOR POSITION [-] • 'POSIITON' shows Setting position. (ANALYSIS, BUBBLING, OPEN). 1023 BUBBLING • When 'Full Specifications' are not connected, 'WEIGHT', 'VALVE' show '---'. • 'VALVE' items show 'Setting positions' (ANALY, PURGE). Second Monitor screen △...

  • Page 125: Setup: Option

    SETUP process 4a. Setting OPTION Use/Unuse UTLTY ＋ Displays present POWER ON (SETUP) Setting contents. SETUP monitor screen SETUP: OPTION CONTRAST S.NO. 02AB-111 ENT key SETUP ITEMS (USE OPTION=1, CONTRAST =2) 1 ENT USE OPTION (NO USE=0, USE=1) 0 ENT SETUP: OPTION CONTRAST S.NO.

  • Page 126: Use

    4b. Setting LCD contrast UTLTY ＋ POWER ON (SETUP) Displays present Setting contents. SETUP: OPTION CONTRAST S.NO. 02AB-111 ENT key SETUP ITEMS (USE OPTION=1, CONTRAST =2) 2 ENT LCD CONTRAST(1 – 7) 1 ENT SETUP: OPTION CONTRAST S.NO. Displays Setting NO USE 02AB-111 contents.
  • Page 127 Remaining volume sensor 0 point adjustment (ZERO ADJ) ZERO ADJ ZERO (B1=1, B2=2, B3=3, B4=4, B5=5, B6=6 _1 R1=7, R2=8, R3=9, WASH=10, WASTE=11) 1 ENT Monitor screen UTILITY 6a. Setting Leak sensor UTLTY UTILITY (LEAK SENSOR=1, BUBBLING TIME=2, ERROR OUT=3, WEIGHT FACTOR=4) 1 ENT LEAK SENSOR (OFF=0, ON=1) 1 ENT...
  • Page 128 6b. Setting N2 gas auto purge time UTLTY UTILITY (LEAK SENSOR=1, BUBBLING TIME=2, ERROR OUT=3, WEIGHT FACTOR=4) 2 ENT BUBBLING TIME (1-120) min 1 ENT Monitor screen Setting Error out contact UTLTY Error that outputs Error contact in ENABLE • Leak error (1-5) UTILITY (LEAK SENSOR=1, BUBBLING TIME=2, •...

  • Page 129: Ent

    6d. Setting Weight sensor correction factor UTLTY UTILITY (LEAK SENSOR=1, BUBBLING TIME=2, ERROR OUT=3, WEIGHT FACTOR=4) 4 ENT FACT (B1=1, B2=2, B3=3, B4=4, B5=5, B6=6 _1 R1=7, R2=8, R3=9, WASH=10, WASTE=11) 1 - 11 ENT WEIGHT FACTOR ** (0.800 - 1.200) 1.000 1.000 ENT Monitor screen...
  • Page 130 GLP Function 7a. VALVE1 Report GLP (LOGBOOK=1, KEY LOCK=2) 1 ENT PURGE VALVE SELECT (VALVE1=1, VALVE2=2) 1 ENT 1 ENT LOGBOOK (REPORT=1, RESET=2) 2 ENT VALVE1 SWITCHING LAST CHANGED DATE 23 times 2/25/2000 VALVE1 SEAL CHANGE(MM DD YYYY) <1 - 12> 6 2 2000 6 ENT VALVE1 SEAL CHANGE(MM DD YYYY)
  • Page 131 7b. VALVE2 Report GLP (LOGBOOK=1，KEY LOCK=2) 1 ENT PURGE VALVE SELECT (VALVE1=1, VALVE2=2) 2 ENT 1 ENT LOGBOOK (REPORT=1，RESET=2) 2 ENT VALVE2 SWITCHING LAST CHANGED DATE 23 times 2/25/2000 VALVE2 SEAL CHANGE (MM DD YYYY) 2 2000 <1 - 12> 6 ENT VALVE2 SEAL CHANGE (MM DD YYYY) Monitor screen...
  • Page 132 7c. Key-lock On/Off GLP (LOGBOOK=1, KEY LOCK=2) 2 ENT KEY LOCK (YES=1, NO=0) 1 ENT Monitor screen Purge Valve1 switching VALVE1 PURGE VALVE1 POSITION (ANALYSIS=1, PURGE=2) 2 ENT MOVING Displays while moving (for 2 sec.). Monitor screen 4 - 44...
  • Page 133 Option Valve 2 switching VALVE2 PURGE VALVE2 POSITION (ANALYSIS=1, PURGE=2) 2 ENT MOVING Displays while moving (for 2 sec.). Monitor screen 10. N gas solenoid valve setting (GAS POS) GAS POS POSITION (ANALYSIS=1, BUBBLING=2, OPEN=3) 2 ENT Monitor screen 4 - 45...

  • Page 134: Flow Path Diagram

    4.8 FLOW PATH DIAGRAM 4 - 46...
  • Page 135 Tube connection Tube Tube connection way Kind of tube mark mark 0.25TT Teflon tube 1.0 × 0.5T 0.33TT Teflon tube 0.33 × 0.62T 0.5TT Teflon tube 0.5 × 0.5T 1.0TT Teflon tube 1.0 × 0.5T 1.5PT Polymer tube 1.5 × 0.75T 1.0PT Polymer tube 1.0 ×...

  • Page 136: Wiring Diagram Between Pc Boards

    4.9 Wiring Diagram between PC Boards W-lamp energy Motor for moving right-left LAMP Photointerrupter for moving right-left PI assembly W-lamp Motor for moving front-rear SPD PC board assembly Photointerrupter for moving front-rear Motor for moving up-down MCIF2 PI assembly MR Cable AS IO assembly Photointerrupter for moving up-down LOB PI...

  • Page 137: Circuit Diagram

    4.10 CIRCUIT DIAGRAM Memory unit (S-RAM/EEPROM) LCD control unit LED control unit LCD contrast control unit Key, Buzzer control unit RS-422 circuit CPU (SH-2) circuit 3.3 V circuit gas solenoid valve control unit Contact input circuit Circuit Diagram of ANNEX_1 PC Board (1/2) 4 - 49...
  • Page 138 Circuit Diagram of ANNEX_1 PC Board (2/2) 4 - 50...
  • Page 139 PUMPC 4 - 51...
  • Page 140 PUMPR 4 - 52...
  • Page 141 AS-CONT1 4 - 53...
  • Page 142 AS-CONT2 4 - 54...
  • Page 143 AS-IO 4 - 55...
  • Page 144 L89CO_1 4 - 56...
  • Page 145 L89CO_2 4 - 57...
  • Page 146 L89RA_1 4 - 58...
  • Page 147 L89RA_2 4 - 59...
  • Page 148 MCIF2_1 4 - 60...
  • Page 149 MCIF2_2 4 - 61...
  • Page 150 AID_DC5V 4 - 62...
  • Page 151 Heater current control circuit Peltier current control circuit 5 V circuit ±12 V for ANNEX ±12 V for Oven Circuit Diagram of Oven RP PC Board 4 - 63...
  • Page 152 Module Circuit Table PC board which uses Module Circuit ANNEX PUMPC PUMPR AS-CONT AS-IO COOL CO2 CNT Name of (OVEN RP) (LOG) 28526300 28907301 38907302 28907350 38907351 28907355 28907363 Module 38526332 38526345 38907967 38907967 38907967_1 8106991A 38907967_1 KEYIF 39864385 39864335 39864335 39864385 39864385...
  • Page 153 4 - 65...
  • Page 154 4 - 66...
  • Page 155 4 - 67...
  • Page 156 Circuit Diagram of DC 4 - 68...
  • Page 157 4 - 69...
  • Page 158 4 - 70...
  • Page 159 Gain adjustment circuit Zero adjustment circuit Circuit Diagram of N 4 - 71...
  • Page 160 4 - 72...
  • Page 161 SH-2 Micro-computer (28 MHz) When +15 V for digital circuit becomes 4.3 V or lower, Reset signal is outputted to SH-2 microcomputer. Circuit Diagram of OB2 Module 4 - 73...
  • Page 162 4 - 74...
  • Page 163 4 - 75...
  • Page 164 Circuit Diagram of OFG Module 4 - 76...
  • Page 165 4 - 77...
  • Page 166 Circuit Diagram of OK0 Module 4 - 78...
  • Page 167 Circuit Diagram of OK1 Module 4 - 79...
  • Page 168 4 - 80...
  • Page 169 DC - DC converter (input: +5V, output: +3.3 V) Circuit Diagram of OKR Module 4 - 81...
  • Page 170 4 - 82...
  • Page 171 CPU (including ISP) 24 V Voltage drop detection RES Signal output FPGA (Field programmable gate array) Circuit Diagram of OP1 Module 4 - 83...
  • Page 172 4 - 84...
  • Page 173 DC - DC converter (input: +24 V, output: ±12 V) Circuit Diagram of OT1 4 - 85...
  • Page 174 Circuit Diagram of OT1 4 - 86...
  • Page 175 DC - DC converter (input: +24 V, output: ±5 V) Circuit Diagram of OT2 4 - 87...
  • Page 176 4 - 88...
  • Page 177 Differential input '+' side Differential input '-' side Circuit Diagram of OT4 4 - 89...
  • Page 178 DC - DC converter (input: +24 V, output: ±12 V) Circuit Diagram of OT5 4 - 90...
  • Page 179 4 - 91...
  • Page 180 High withstanding voltage low ion resistance Parity MOS FET array (4 elements incorporated) Circuit Diagram of OT7 4 - 92...
  • Page 181 4 - 93...
  • Page 182 4 - 94...
  • Page 183 DC - DC converter (input: +24 V, output: ±12 V) Circuit Diagram of OT8 4 - 95...
  • Page 184 Circuit Diagram of OTB 4 - 96...
  • Page 185 Circuit Diagram of OTC 4 - 97...
  • Page 186 Low ion resistance, power MOS-FET OTDQ2 On makes OTDQ1 On Because of high resistance, malfunction of OKE Module circuit caused by voltage generated motor movement by external activation when the instrument power supply is off is prevented. Circuit Diagram of OTD 4 - 98...
  • Page 187 4 - 99...
  • Page 188 4 - 100...
  • Page 189 Circuit Diagram of OTE 4 - 101...
  • Page 190 DC - DC converter (input: +24 V, output: +5 V) Circuit Diagram of OU7 4 - 102...
  • Page 191 4 - 103...

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